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Rosie Galligan and Lark Atkin-Davies will miss England’s Grand Slam decider against France on Saturday through injury.
Lock Galligan, 25, pulled out of the 88-10 win over Ireland in the warm-up with a thumb injury, while hooker Atkin-Davies, 29, came off with an ankle injury.
Both will see specialists this week to assess the injuries.
Exeter Chiefs wing Katie Buchanan has been called up after injury, while Gloucester-Hartpury number eight Sarah Beckett and Leicester hooker Amy Cokayne are available after bans.
Victory in Bordeaux would secure a third successive Grand Slam and sixth Six Nations title in a row for the Red Roses.
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